产品属性：

商品介绍：

英文名称PCNA Mouse mAb, Nuclear Loading Control

中文名称增殖细胞核抗原（内参）单克隆抗体

别    名Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen  

产品类型内参抗体

研究领域肿瘤  细胞生物  细胞周期蛋白  转录调节因子  细胞类型标志物  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号10E9

交叉反应Human,Mouse,Rat

产品应用WB=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-200

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量29 kDa

检测分子量

细胞定位细胞核

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human PCNA

亚    型IgG

纯化方法affinity purified by Protein G

缓 冲 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed
PCNA Mouse mAb, Nuclear Loading Control产品介绍Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.