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Echothiophate is an organophosphate and acetylcholinesterase (AChE) inhibitor. It is more active at ocular acetylcholinesterases than those isolated from rabbit brain, liver, or blood. In vivo, topical ocular administration of echothiophate (0.125 and 0.25% w/v) reduces intraocular pressure and induces miosis in dogs with normotensive eyes and those with inherited glaucoma. It also induces formation of subcapsular cataracts in cynomolgus monkeys. Formulations containing echothiophate have been used for the treatment of glaucoma.

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