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IC50: N/A

DPNI-GABA, also known as Nitroindoline-caged GABA, has similar photo with MNI-glutamate, including the same quantum yield (0.085), highly water soluble, exhibitting fast photorelease that is efficient at near-UV and 405 nm wavelengths.

In vitro: Under the condition of retaining the advantages of nitroindoline cages, DPNI-GABA were modified to minimize the pharmacological interference commonly found with caged GABA reagents. Unlike previous test of the 5-methoxycarbonylmethyl-7-nitroindolinyl-GABA, DPNI-GABA reduced peak GABA-evoked responses with an IC50 of approximately 0.5mM, and blocked GABAA receptors with much lower affinity,. It is most important that the kinetics of receptor activation, comparable to synaptic events, were determined as 10–90% rise-times, and had no effect by DPNI-GABA present at 1 mM concentration, and permitted photolysis of DPNI-GABA to mimic synaptic activation of GABAA receptors. As estimated, the spatial resolution of uncaging DPNI-GABA in dendrites was 2m laterally and 7.5m focally with a laser spot of 1mapplied to cerebellar molecular layer interneurons. Finally, photorelease restricted to the area of the soma inhibited spiking in single Purkinje neurons or molecular layer interneurons for periods regulated by the flash intensity and duration at low DPNI-GABA concentration [1,2].

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