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Quinacrine mustard is a fluorescent DNA-intercalating agent.1 It selectively binds to adenine-thymine (AT) base pairs over guanine-cytosine (GC) base pairs.2,3 Quinacrine mustard has been used to label metaphase chromosomes for karyotyping by autoradiography.1 It displays excitation/emission maxima of 425/480 nm, respectively.4

|1. Caspersson, T., Farber, S., Foley, G.E., et al. Chemical differentiation along metaphase chromosomes. Exp. Cell Res. 49(1), 219-222 (1968).|2. Ellison, J.R., and Barr, H.J. Quinacrine fluorescence of specific chromosome regions. Late replication and high A:T content in Samoaia leonensis. Chromosoma 36(4), 375-390 (1972).|3. Weisblum, B., and De Haseth, P.L. Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylate-rich regions in DNA. Proc. Natl. Acad. Sci. U.S.A. 69(3), 629-632 (1972).|4. Chen, R.F. Fluorescence of quinacrine mustard conjugated to proteins. Arch. Biochem. Biophys. 172(1), 39-50 (1976).

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