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抑制剂 > 细胞周期/检查点 > Enteropeptidase Fluorogenic Substrate (trifluoroacetate salt
产品名称:
Enteropeptidase Fluorogenic Substrate (trifluoroacetate salt
型号:
CS-01Y73725
生产地址
进口、国产
产品价格
电议
产品简介
质量保证、价格优惠
产品详情

一、概述:

化学性质:                                                                                                             

规格

500 µg 1 mg 5 mg

CAS

N/A

别名

N/A

化学名

N/A

分子式

C34H40F3N7O16·XCF3COOH

分子量

859.7

溶解度

DMF: 30 mg/mL,DMSO: 30 mg/mL,Ethanol: 10 mg/mL,PBS (pH 7.2): 2.5 mg/mL

储存条件

-20°C

General tips

For obtaining a higher solubility , please warm the tube at 37 and shake it in the ultrasonic bath for a while.

Shipping Condition

Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

 

产品描述:                                                                                                            

Enteropeptidase fluorogenic substrate is a substrate for enteropeptidase that contains a 7-amino-4-trifluoromethylcoumarin (AFC) moiety. Enteropeptidase is a serine protease expressed in the proximal small intestine of higher animals that converts inactive trypsinogen to active trypsin by endoproteolytic cleavage.1,2 Enteropeptidase recognizes the highly specific amino acid sequence DDDDK on the fluorogenic substrate and cleaves after the lysine residue, releasing the AFC moiety. Enteropeptidase activity is quantified by fluorescent detection of AFC, which displays excitation/emission spectra of 380/500 nm.3

1.Pepeliaev, S., Krahulec, J.,  ernÝ, Z., et al.High level expression of human enteropeptidase light chain in Pichia pastorisJ. Biotechnol.156(1)67-75(2011) 2.Choi, M.-G., Lee, S., Chung, H.-S., et al.A fluorogenic method for measuring enteropeptidase activity: Spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarinBMB Rep.44(7)458-461(2011) 3.Gossrau, R.Cytochemistry of membrane proteasesHistochem J.17(7)737-771(1985).

特别提醒:                                                                                                              

1. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。

2. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。

标签:Enteropeptidase Fluorogenic Substrate (trifluoroacetate salt) 

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