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G-418 is an elongation inhibitor of 80 S ribosomes [1].

G418 is an aminoglycoside antibiotic, it has a wide spectrum of activity and is active against bacteria, yeasts, fungi, algae, plant and even animal cells. G418 is usually used as a cloning selection in pro- and eukaryotes at concentrations from 1 to 300μg/ml. In the studies of antibiotic resistance in clinical isolates of bactenia, it is found that all strains carrying 3'-O-aminoglycoside phosphotransferases are resistant to G418. Among these phosphotransferases, type I and type II are encoded by the transposable elements TnS and Tn601, respectively [2].

Apart from the selector function, G418 is also found to have antiviral activity against bovine viral diarrhea virus (BVDV). G418 prevents the cytopathic effect (CPE) resulting from DENV-2 infection of BHK cells, in a dose-dependent manner with an EC50 value of 3±0.4μg/ml [3].

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