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Hygromycin B-d4 is intended for use as an internal standard for the quantification of hygromycin B by GC- or LC-MS. Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus.1,2 It is active against E. coli (MIC = 150 µg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis.3,4,5 Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph.4,5

1.Eustice, D.C., and Wilhelm, J.M.Mechanisms of action of aminoglycoside antibiotics in eucaryotic protein synthesisAntimicrob. Agents Chemother.26(1)53-60(1984) 2.CabaÑas, M.J., VÁzquez, D., and Modolell, J.Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognitionEur. J. Biochem.8721-27(1978) 3.McGaha, S.M., and Champney, W.S.Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coliAntimicrob. Agents Chemother.51(2)591-596(2006) 4.Kaster, K.R., Burgett, S.G., Rao, R.N., et al.Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencingNucleic Acids Res.11(19)6895-6911(1983) 5.Blochlinger, K., and Diggelmann, H.Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cellsMol. Cell Biol.4(12)2929-2931(1984)

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