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CEP-1347, also called KT 7515, is an inhibitor of the c-Jun N-terminal kinase (JNK) signaling pathway, with an IC50 value for JNK1 activation of 20 ± 2 nM in rat embryonic motoneurons [1].

The JNK pathway, also known as the stress-activated protein kinase (SAPK) pathway, is one of the signaling cascades that mediate the apoptotic death in response to a variety of stressful stimuli. JNK activation by phosphorylation is important for neuronal cell death after injury in vivo and after trophic factor withdrawal in vitro [2].

CEP-1347 induced neuronal survival. JNK1 activity in untreated cell cultures increased approximately fourfold within 24 hr after plating. As early as 15 min after the application of CEP-1347 at 500 nM, the activity of JNK1 sharply decreased to ~50% of control levels. For the next 24 hr, the activity of JNK1 continued to decrease. Cultures rich in motoneurons were grown in the presence of CEP-1347 at increasing concentrations, and the IC50 for JNK1 activity at 22 hr was 21 ± 2 nM, whereas the EC50 for cell survival at 5 d was 20 ± 2 nM [1].

CEP-1347 can affect noise-induced hearing loss. Data showed that hearing thresholds 2 d before noise exposure showed no significant difference between the noise-exposed control and treated group. Hearing threshold shifts in all guinea pigs 2 d after the noise exposure. By day 6 after exposure, threshold shifts were significantly less in the CEP-1347 group than in the noise-exposed control group. By 2 weeks after exposure, the difference between the two groups became more pronounced [2].

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