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Vitamin D3-d7 is intended for use as an internal standard for the quantification of vitamin D3 by GC- or LC-MS. Vitamin D3 is a biologically inactive precursor to calcitriol that is converted to active metabolites in vivo. Vitamin D3 is obtained from dietary sources, including fish, or formed in the epidermis via photolytic conversion of 7-dehydro cholesterol to previtamin D3 by UVB radiation, followed by isomerization to vitamin D3. Vitamin D3 can then be converted to 25-hydroxy vitamin D3 in the liver by the cytochrome P450 (CYP) isoform CYP2R1 before being converted to calcitriol by CYP27B1 in the kidney. Formulations containing vitamin D3 have been used in the treatment of osteoporosis.

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