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Isolithocholic acid is a bile acid that is formed via microbial metabolism of lithocholic acid or lithocholic acid 3α-sulfate .[1],[2] Isolithocholic acid (0.01%) inhibits spore germination induced by taurocholic acid in the C. difficile strains CD196, M68, BI9, and 630, as well as inhibits growth and decreases the cytotoxicity of C. difficile culture supernatants to Vero cells when used at a concentration of 0.0003%.[3] Fecal levels of isolithocholic acid are decreased in a rat model of high-fat diet-induced obesity compared with rats fed a normal diet.[4]

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