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Methylmalonyl coenzyme A (methylmalonyl-CoA) is an intermediate in multiple metabolic pathways in bacteria and eukaryotes.1,2,3 It is an intermediate in carbon assimilation in certain bacteria and carbon fixation in plants.1,2 Methylmalonyl-CoA is converted to succinyl-CoA by methylmalonyl-CoA mutase with vitamin B12 as a coenzyme.3 A deficiency in vitamin B12 leads to a build-up of methylmalonyl-CoA.4

1.Anthony, C.How half a century of research was required to understand bacterial growth on C1 and C2 compounds; the story of the serine cycle and the ethylmalonyl-CoA pathwaySci. Prog.94(Pt 2)109-137(2011) 2.Tabita, F.R.The hydroxypropionate pathway of CO2 fixation: Fait accompliProc. Natl. Acad. Sci. U.S.A.106(50)21015-21016(2009) 3.Medicine, I.o.Vitamin B12Dietary reference intakes for thiamin, riboflavin, niacin, vitamin B6, folate, vitamin B12, pantothenic acid, biotin, and choline306-356(1998) 4.Cardinale, G.J., Carty, T.J., and Abeles, R.H.Effect of methylmalonyl coenzyme A, a metabolite which accumulates in vitamin B12 deficiency, on fatty acid synthesisJ. Biol. Chem.245(15)3771-3775(1970)

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