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4-Nitrophenyl-N-acetyl-β-D-glucosaminide is a chromogenic substrate for N-acetyl-β-glucosaminidase.1,2 Hydrolysis of 4-nitrophenyl-N-acetyl-β-D-glucosaminide by N-acetyl-β-glucosaminidase releases 4-nitrophenol, which can be quantified by colorimetric detection at 405 nm as a measure of N-acetyl-β-glucosaminidase activity. It has been used to quantify the activity of N-acetyl-β-D-glucosaminidase in human serum and urine in parenterally fed patients.3

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