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BRL 50481 is a selective inhibitor of phosphodiesterase (PDE) 7. At substrate concentrations of 50 nM and 1 ?M, the hydrolysis of cAMP by hrPDE7A1 was inhibited by BRL 50481 with IC50 values of 0.26 and 2.4 ?M, respectively [1]. BRL 50481 is a selective and potent inhibitor of human recombinant PDE7A with an IC50 value of 26 nM [2].

PDE7 is of a high affinity as a cAMP-specific PDE. PDE7 could be a good treatment target for T cell related diseases, airway diseases or central nervous system (CNS) disorders [3].

At 1 ?M and 50 nM cAMP, BRL 50481 was a selective inhibitor of hrPDE7A1, with 416 and 1884 times less potencies against PDE3, and 38 and 238 times less potencies against hrPDE4A4, respectively. BRL 50481 did not significantly inhibit PDE1C, PDE1B, PDE5 and PDE2 at concentrations below 100 ?M. BRL 50481 showed a competitive inhibition potency against hrPDE7A1 with a Ki value of 180±10 nM. But in MOLT-4 T cells, BRL 50481 increased the cAMP content with an EC50 >> 100 ?M. The EC80 value of rolipram is about 10 ?M. When the effect of BRL 50481 at concentrations of 10–300 ?M on cAMP mass was examined in the presence of rolipram at a submaximal concentration, the mean concentration-response curve of BRL 50481 was displaced upwards of a significantly greater magnitude, compared to the sum of the cAMP response affected by BRL 50481 and rolipram alone [1].

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